![]() ![]() ![]() # of samples, events and stains, species and cell type, etc.) and this can help FlowJo perform optimally. You can set it up according to your general specifications (e.g. The core of the program is based around its workspace (Figure 1) and this is where you add all your samples, create various gates and statistics, and set up groups, etc. Analyzing Your Data with FlowjoĮssentially FlowJo is solely analysis software, though it does run acquisition on reconditioned cytometers by Cytek Development (“ FlowJo Collectors’ Edition”). ![]() ![]() We are starting this series off with FlowJo, which has become very popular, in particular among many in the immunology world who may have large complex datasets. In this series of articles we are going to be looking at a range of software that is available to you for your cytometric analysis and give you the low down on each one, highlighting the strengths and weaknesses so you can make the decision of which software is best for you. So where we use the dedicated software aligned to the analyser for acquisition, we frequently use alternative programs for offline analysis.Īdvantages may be that such software is much more user-friendly for large scale analysis rather than the instrument-linked software (although there are usually analysis tools here too), and it can unify the analysis approach and presentation, so irrespective of what user or cytometer, the data “looks” the same.īut with the variety of software available how do you know which one is right for you? This can be increasingly complex depending on the number of cells, populations, parameters, and combinations. Ours offers FlowJo X dongles for you to run your own FlowJo data analysis on your own personal computer, so you don't need to spend $$ for good software.Generally, once you’ve acquired your samples on a cytometer, the hard (but hopefully fun!) part of making sense of all the data begins. You should also check out your local core facility. Summit was free if you have Coulter equipment, though the deal may have changed - Coulter seems to push Kaluza more. The last time this question came up, the following software was mentioned:įlowing Software - Purdue has Cytospec. This question comes up an awful lot on the purdue mailing list. ![]()
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